Phloxin B Acid Red 92 Uses and Applications Guides
Author : Pradip Naskar | Published On : 15 Sep 2026
Phloxin B is a bright red synthetic dye known for its distinctive colour, water solubility, and usefulness in laboratory and industrial applications. It belongs to the xanthene family of dyes and is widely recognised by its Colour Index name Acid Red 92. Because of its strong colouring properties and suitability for specialised applications, this dye is used in areas such as biological staining, laboratory research, and selected colouration processes.
For businesses and laboratories that require consistent dye performance, understanding the chemical identity, properties, applications, and quality considerations of the product is important. This guide explains the essential information about Phloxin B and the factors that should be considered when selecting it for a particular application.
What Is Phloxin B
Phloxin B is a synthetic xanthene dye with a strong red appearance. It is identified by CAS number 18472 87 2 and Colour Index number 45410. Its chemical name and structure are associated with a brominated and chlorinated fluorescein derivative in disodium salt form.
The product is commonly supplied as a powder and is valued for its intense colour and water solubility. Depending on the intended application, different grades may be required to achieve the desired level of purity and performance.
The product is also commonly identified as Acid Red 92 in technical and commercial settings.
Chemical Identity and Key Properties
Understanding the chemical characteristics of a dye helps manufacturers and laboratory professionals select the right grade for their requirements.
According to Emichem product information, the product has the following characteristics:
• CAS number 18472 87 2
• Colour Index number 45410
• Colour Index generic name Acid Red 92
• Molecular formula C20H2Br4Cl4Na2O5
• Molecular weight approximately 829.6 g/mol
Different grades listed by Emichem include Supra, Crude, and Extra Pure grades. The Extra Pure grade is described as providing a transparent and clear solution, with heavy metal content maintained within prescribed limits.
These characteristics can be particularly important for laboratory applications where clarity, purity, and consistent staining performance are required.
Why Phloxin B Is Important
The value of a specialised dye is not limited to its colour. Its chemical structure and interaction with different materials make it useful in specific technical applications.
Phloxin B is particularly recognised in microscopy and biological staining. It can help provide strong visual contrast when used as part of staining procedures. Scientific literature also identifies it as a xanthene dye used in microtechnique and biomedical research.
Its strong red colour makes it useful where researchers need to distinguish particular cellular structures or tissue components. The exact staining result depends on the formulation, concentration, staining procedure, and other reagents used.
Applications of Phloxin B
One of the main reasons for the commercial importance of this dye is its range of specialised applications.
Biological and Histological Staining
Phloxin B is used as a biological stain in laboratory and microscopy applications. It can provide red coloration to selected structures during staining procedures, helping researchers examine tissue and cellular components under a microscope.
It is also associated with hematoxylin phloxine saffron staining methods, where it contributes red coloration to cytoplasm and connective tissue.
Laboratory Research
Specialised dyes are important tools in research because colour can make specific structures easier to observe. Phloxin B can therefore be used in laboratory procedures where a strong red stain is required.
The grade of dye should be selected according to the requirements of the laboratory procedure. Higher purity grades may be preferred for applications where impurities could affect solution clarity or experimental consistency.
Microscopy Applications
Microscopy is another important area where this dye is used. Commercial laboratory suppliers list Phloxin B specifically for microscopy applications. It can be incorporated into staining procedures designed to highlight cellular structures and provide visual differentiation.
Research and Scientific Analysis
Because xanthene dyes have distinctive optical properties, they can be useful in scientific research involving colour and fluorescence based observations. Reported technical data for Phloxin B includes absorption characteristics in the visible range, although the exact optical behaviour depends on the solvent and measurement conditions.
Factors to Consider When Choosing a Grade
Selecting the right dye grade is important for achieving consistent results. Buyers should consider several factors before placing an order.
Purity
Purity is one of the most important considerations, particularly for laboratory and research applications. A higher purity material can help reduce unwanted interference in specialised procedures.
Dye Content
The actual dye content can influence colour strength and application performance. Buyers should review the technical specification and certificate of analysis supplied for the selected grade.
Solution Clarity
For applications requiring prepared dye solutions, clarity can be an important consideration. A suitable grade should produce the expected solution characteristics under the recommended preparation conditions.
Heavy Metal Specifications
For laboratory and specialised applications, buyers should also review heavy metal specifications and other relevant quality parameters. Emichem states that its Phloxin B grades maintain heavy metal content within prescribed limits.
Consistency Between Batches
Industrial and laboratory users often require consistent results from one purchase to another. Working with an established manufacturer can make it easier to maintain consistent specifications across different batches.
How to Select a Reliable Phloxin B Supplier
Choosing a supplier involves more than comparing prices. The supplier should be able to provide appropriate technical information, product specifications, and quality documentation.
Before purchasing, businesses should consider:
• Product purity and dye content
• Available grades
• CAS and Colour Index identification
• Certificate of analysis availability
• Packaging requirements
• Batch consistency
• Technical support
• Storage and handling recommendations
• Delivery capability
A supplier with experience in specialised dyes can also help customers identify the appropriate grade for their intended application.
Phloxin B from Emichem
Emichem Pvt Ltd. offers Phloxin B in different grades, including Supra, Crude, and Extra Pure. The company identifies the product as Acid Red 92 and provides technical information including its CAS number, Colour Index number, molecular formula, and molecular weight.
For buyers looking for specialised dyes, reviewing the available grades and technical specifications before purchase can help ensure that the selected product matches the intended application.
Storage and Handling Considerations
Proper storage is important for maintaining the quality of powdered dyes. The product should be stored according to the manufacturer's technical and safety recommendations. Containers should remain properly closed when not in use, and the material should be protected from unsuitable environmental conditions.
Users should always consult the applicable safety data sheet before handling the product, particularly in laboratory or industrial environments. Appropriate personal protective equipment and workplace procedures should be followed according to the application and local requirements.
Conclusion
Phloxin B is a specialised synthetic xanthene dye with an important role in biological staining, microscopy, laboratory research, and other technical applications. Its identification as Acid Red 92, along with its strong red colour and useful chemical properties, makes it a valuable material for applications requiring reliable and distinctive staining performance.
Choosing the correct grade is essential for achieving consistent results. Factors such as purity, dye content, solution clarity, heavy metal specifications, documentation, and batch consistency should all be considered before purchase. By selecting a suitable grade from a reliable manufacturer, laboratories and businesses can obtain a dye that meets their specific technical requirements.
