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Best RNAscope multiplex

Author : Denovo Technologies | Published On : 04 Dec 2023

A method for identifying and seeing RNA molecules inside of cells or tissues is called RNAscope Multiplex. Multiple RNA targets may be detected simultaneously in a single sample thanks to this extremely sensitive and specific approach. The RNAscope technique operates on the basis of in situ hybridization, a process in which RNA probes bind to complementary RNA targets within the sample. A particular probe tagged with distinct oligonucleotide sequences is used to identify each RNA target.

 


 

 

Using several probes tagged with distinct oligonucleotide sequences, the RNAscope Multiplex technology allows for the simultaneous detection of numerous RNA targets. These probes may be made to target various RNA molecules, such as non-coding RNA or mRNA, enabling researchers to look into the relationships and patterns of numerous RNA molecules' expression.

The extremely specific nature of the probes utilized in RNAscope Multiplex allows for accurate single-cell localization of RNA targets. Bright and distinct signals are produced as a result of the signal amplification process, which enables the identification and visualization of the signals via brightfield imaging or fluorescence microscopy.

Numerous scientific disciplines, including molecular biology, cancer research, neurology, developmental biology, and pathology, make extensive use of RNAscope Multiplex. By enabling the simultaneous identification of several RNA targets inside intricate biological samples, it offers insightful information about cellular relationships, gene expression patterns, and disease causes.

 

Importance of RNAscope Multiplex for Denovotec 

RNAscope Multiplex is a key component of Denovotec's products.  The purpose of the RNAscope Multiplex test is to facilitate the geographic investigation of gene expression in diverse and complicated tissues, including the brain, cancers, and growing organs. Simultaneous detection of many RNA targets at the single-cell level makes it an effective tool for large-scale investigations.

One may comprehend the significance of RNAscope Multiplex by examining its primary attributes and advantages:

  1. Sensitivity: Gene expression analysis and detection using conventional RNA in situ hybridization (RNA-ISH) methods can be difficult due to their frequently low sensitivity. The remarkable sensitivity of RNAscope Multiplex assays makes it possible to identify individual RNA molecules in targets with low abundance.

  2. Plexing Capability: RNAscope Multiplex assays offer different plexing levels, enabling the simultaneous detection of many genes. This skill is essential for recognizing and describing many cell types and subtypes in intricate tissues, which advances our knowledge of cellular heterogeneity.

  3. Cell-Specific Marker Identification: Using cell-specific markers, the RNAscope Multiplex test makes it possible to identify individual cells. This capability makes it possible for researchers to accurately identify and examine particular cell populations inside tissues, which enables more focused and thorough study.

  4. Comprehensive examination: RNAscope Multiplex assays offer a thorough examination of gene expression patterns by verifying cells through the simultaneous detection of other targets. Researchers may learn more about the connections and interactions between many genes in cells and tissues thanks to this thorough method.

  5. Histological Context: A comprehensive knowledge of cell biology requires the integration of single-cell RNA sequencing (scRNA-seq) data with histological context. A useful tool for examining gene expression patterns in the context of their anatomical surroundings is the ability to see different cell types using scRNA-seq data in situ using RNAscope Multiplex assays.

For the purpose of analyzing gene expression in space, Denovotec's RNAscope Multiplex assay is an effective and adaptable instrument. It makes it easier for scientists to examine intricate tissues, recognize certain cell types, and get a thorough grasp of gene expression patterns.